Bystander Host Cell Killing Effects of Clostridium perfringens Enterotoxin
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Bystander Host Cell Killing Effects of Clostridium perfringens Enterotoxin
Clostridium perfringens enterotoxin (CPE) binds to claudin receptors, e.g., claudin-4, and then forms a pore that triggers cell death. Pure cultures of host cells that do not express claudin receptors, e.g., fibroblasts, are unaffected by pathophysiologically relevant CPE concentrations in vitro However, both CPE-insensitive and CPE-sensitive host cells are present in vivo Therefore, this study...
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A rapid assay which measured the biological activity of Clostridium perfringens enterotoxin was developed. The method involved the rapid killing of Vero cells by enterotoxin produced by C. perfringens grown in Duncan and Strong sporulation medium. Serial dilutions of toxin were added to Vero cells either in suspension or grown as monolayers in wells of a 96-well cell tissue culture cluster plat...
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Anti-enterotoxin immunoglobulins immobilized on CH-Sepharose or CNBr-Sepharose were used for affinity chromatography purification of Clostridium perfringens enterotoxin. Cell extracts containing enterotoxin or partially purified toxin preparations were applied to the column and nonspecifically-bound protein was eluted. NaOH was used to elute specifically bound toxin. The purity of enterotoxin p...
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متن کاملClostridium perfringens Sporulation and Enterotoxin Synthesis
5 Running title: Role of SigF and SigG in sporulation and CPE synthesis 6 * To Whom Correspondence Should be Addressed: 7 Bruce A. McClane 8 Department of Microbiology and Molecular Genetics 9 447 Bridgeside Point II Building 10 450 Technology Drive 11 University of Pittsburgh School of Medicine 12 Pittsburgh, PA 15219 13 Phone: 412-648-9022 14 Fax: 412-624-1401 15 Email: [email protected] 16 Copy...
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ژورنال
عنوان ژورنال: mBio
سال: 2016
ISSN: 2161-2129,2150-7511
DOI: 10.1128/mbio.02015-16